Contract with Fourmentin-Guilbert Foundation: Macromolecule detection in 3D cellular cryo-electron tomograms

Participants: Emmanuel Moebel, Charles Kervrann.

Duration: 5 months (Dec 2019 – Apr 2020).

The objective of the project is to improve the DeepFinder software dedicated to the detection and identification of macromolecules within 3D cellular cryo-electron tomograms. In collaboration with Fourmentin-Guilbert Foundation, the goal is to build cellular atlases of several organisms from localizations of macromolecules (see Software DeepFinder in Section 6.9).

Funding: Fourmentin-Guilbert Foundation.

Collaborators: D. Larivière & E. Fourmentin (Fourmentin-Guilbert Foundation), A. Martinez & W. Baumeister (Max Planck Institute, Martinsried, Germany).

Contract with DGA: Motion saliency analysis in videos

Participants: Léo Maczyta, Patrick Bouthemy.

Duration: 36 months (Oct 2017 – Sep 2020).

This project funded by the DGA (Ministry of defense) and Région-Betagne concerns the PhD thesis (co-funding) carried out by Léo Maczyta. The goal is to develop motion saliency methods along three axes: temporal motion saliency detection, saliency map estimation, trajectory-based saliency detection (see Section 7.10).

Funding: DGA (National Defense Agency) and Région-Bretagne.

Contract with GATACA Systems: Super-resolution microscopy and in live cell imaging

Participants: Jean Salamero, Ludovic Leconte, Charles Kervrann.

Duration: 36 months (Jan 2017 – Dec 2019).

The objective of the project is to transfer innovations for Multi-Angle TIRFM (using Azymuthal TIRFM from Ilas2) and collaborate as “β-Test site” for SIM in Nipkow disk microscopy (product: Live-SR).

Funding: GATACA Systems company.

Collaborators: C. Gueudry (GATACA Systems), J. Boulanger (MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, UK).

Contract with CryoCapCell SA: 3D LIVE CLEM (Correlative Light and Electron Microscopy) to decipher fates and functions of exosomes in vivo

Participant: Jean Salamero.

Duration: 24 months (Oct 2018 – Sep 2020).

The objective of the project is to link dynamic biogenesis of intracellular membrane compartments with their ultrastructures. It combines fast high resolution photonic imaging ( MA-TIRFM and fast high pressure freezing for 3D cryoEM. It requires adapted registration methods in 3D, in order to navigate through the multiple scales.

Funding: DIM–ELICIT Empowering LIfe sCiences with Innovative Technologies (Région Ile de France).

Collaborators: G. Van Niel (coordinator, Institute of Psychiatry and Neuroscience of Paris), G. Raposo (CNRS-UMR 144 Institut Curie PSL Research), X. Heiligenstein (CryoCapCell SA).